منابع مشابه
Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.
Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative analysis of macromolecules in solution. AUC has broad applications for the study of biomacromolecules in a wide range of solvents and over a wide range of solute concentrations. Three optical systems are available for the analytical ultracentrifuge (absorbance, interference, and fluorescence) that permi...
متن کاملSedimentation Velocity Analytical Ultracentrifugation
(b) Sedimentation equilibrium: an experiment performed at a lower speed so that the sedimentation and backdiffirsion forces are of comparable magnitudes and therefore give rise to an equilibrium distribution of solute concentration. Because there is no net transport at equilibrium, shape effects do not come into play and the distribution becomes an absolute function of molecular mass for a sing...
متن کاملVariation of sedimentation coefficient with rotor velocity.
The sedimentation coefficients of three proteins, which appeared homogeneous in the ultracentrifuge, have been determined at different rotor velocities. Two of the proteins, ovalbumin and human gamma-globulin, showed sedimentation coefficients that were approx. 10% higher at rotor velocities below 20000rev./min. than at 56100rev./min. The third protein, haemocyanin of Jasus lalandii, could not ...
متن کاملVelocity correlations and diffusion during sedimentation.
We study the dynamics of sedimenting non-Brownian particles under steady-state conditions in two-dimensional geometry. We concentrate on the autocorrelation functions of the velocity fluctuations and the corresponding memory functions and diffusion coefficients as functions of Phi(V) for small but finite Reynolds numbers. For the numerical simulations we have chosen the model of Schwarzer [Phys...
متن کاملIsolation and separation of highly enriched fractions of viable mouse gastric parietal cells by velocity sedimentation
Methods of tissue dissociation and cell separation have been modified to obtain highly enriched fractions of mouse gastric parietal cells. Suspension of gastric mucosal cells are prepared by pronase digestion of the glandular portion of the stomach from adult mice. By utilizing the velocity sedimentation technique to separate cells of different sizes it is possible to recovery parietal cells, w...
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ژورنال
عنوان ژورنال: Nature
سال: 1988
ISSN: 0028-0836,1476-4687
DOI: 10.1038/333097a0